Principal Scientist/Associate Director - siRNA and ASO Gene Silencing - Enigma Search : Job Details

Principal Scientist/Associate Director – siRNA and ASO Gene Silencing Principal Scientist/Associate Director – siRNA and ASO Gene Silencing Get AI-powered advice on this job and more exclusive features.We are seeking a highly motivated and skilled scientist to join our team in the development and application of siRNA and ASO technologies for gene silencing. The successful candidate will be responsible for designing, optimizing, and implementing nucleic acid-based strategies to modulate gene expression for therapeutic purposes. This role requires expertise in molecular biology, RNA therapeutics, and gene silencing mechanisms.Key ResponsibilitiesDesign and Development:- Design siRNA and ASO molecules targeting specific genes to modulate expression through RNA interference (RNAi) or antisense mechanisms.- Optimize sequence specificity to minimize off-target effects while maximizing efficacy.-Mechanism Exploration:- Investigate mechanisms of action for siRNA.- Evaluate mRNA degradation pathways, pre-mRNA splicing alterations, and protein translation inhibition.- Conduct in vitro and in vivo experiments to validate the efficacy of siRNA/ASO molecules.- Use molecular techniques such as qPCR, Western blotting, RNA sequencing, and 5′ RACE to assess target knockdown efficiency.- Perform dose-response studies to determine optimal concentrations.-Therapeutic Applications:- Collaborate on projects aimed at developing siRNA/ASO-based therapies for diseases such as autoimmune, neuroscience, or metabolic diseases.- Explore delivery systems (e.g., nanoparticles, liposomes) to enhance cellular uptake of siRNA.- Investigate chemical modifications of ASOs to improve stability and reduce immunogenicity.-Data Analysis and Reporting:- Analyze experimental data to assess on-target and off-target effects.- Present findings in internal meetings and contribute to patent applications.- Work closely with cross-functional teams including antibody discovery, protein science, biology and pharmacology.- Stay updated on advancements in RNA therapeutics and contribute to strategic planning.Qualifications- Ph.D. degree in Molecular Biology, Biochemistry, Genetics, or a related field with relevant experience.- Strong understanding of siRNA and ASO mechanisms of actions.- Hands-on experience with nucleic acid design tools and molecular biology techniques.- Familiarity with delivery systems for nucleic acids (e.g., lipid nanoparticles).- Knowledge of therapeutic applications of gene silencing technologies.Preferred Skills- Experience with allele-specific RNA silencing strategies.- Expertise in chemical modifications of oligonucleotides for enhanced stability.- Knowledge of regulatory considerations for RNA-based therapeutics.This position offers an exciting opportunity to contribute to cutting-edge research in antibody-siRNA therapeutics by leveraging the power of siRNA and ASOs for precise gene silencingSeniority level Seniority level DirectorEmployment type Employment type Full-timeJob function Job function ScienceReferrals increase your chances of interviewing at Enigma Search by 2xGet notified about new Science Specialist jobs in San Mateo County, CA .Principal/Senior Scientist, Analytical Science San Mateo, CA $140,140.00-$183,690.00 1 week agoResearch Associate (ARD FEL - Attosecond Science) Menlo Park, CA $70,000.00-$100,000.00 10 hours agoMenlo Park, CA $130,000.00-$182,000.00 2 weeks agoMenlo Park, CA $130,000.00-$182,000.00 2 weeks agoResearch Associate (ARD FEL - Attosecond Science) Payment Assurance Specialist, Business Compliance, YouTube San Bruno, CA $137,000.00-$200,000.00 2 weeks agoResearch Associate - LCLS SRD Laser and Chemical Sciences Business Development Senior Specialist – Healthcare & Life Sciences Product Marketing Manager: B2B Life Sciences, Research or Evidence Generation San Bruno, CA $103,000.00-$146,000.00 9 hours agoProduct Marketing Manager: B2B Life Sciences, Research or Evidence Generation San Bruno, CA $128,000.00-$183,000.00 9 hours agoRedwood City, CA $190,000.00-$285,324.00 1 week agoScientist/Senior Scientist, Computational Biology (Contract) South San Francisco, CA $45.00-$52.00 18 hours agoScientist, Discovery Sciences & Technologies Foster City, CA $146,540.00-$189,640.00 1 week ago2025 Fall Intern - Genentech Computational Sciences - Biology Research | AI Development, Machine Learning for Trial Design 2025 Fall Intern - Biology Research | AI Development South San Francisco, CA $150,000.00-$200,000.00 19 hours agoSan Carlos, CA $75,698.00-$113,548.00 1 month agoDaly City, CA $160,000.00-$195,000.00 15 hours agoClinical Science Specialist, Pediatric Liver, Northern California Daly City, CA $120,000.00-$190,000.00 4 days agoResearch Scientist, Demography and Survey Sciences Menlo Park, CA $145,000.00-$204,000.00 3 weeks agoBrisbane, CA $100,000.00-$125,000.00 2 weeks agoResearch Scientist, Demography and Survey Sciences Menlo Park, CA $173,000.00-$242,000.00 2 weeks agoSan Mateo, CA $226,185.00-$292,710.00 10 hours agoManaging Engineer - Thermal Sciences (Ph.D.) Menlo Park, CA $160,000.00-$205,000.00 2 weeks agoPostdoctoral Scientist, Structural Biology Foster City, CA $126,820.00-$164,120.00 1 week agoSenior Machine Learning Scientist (BRAID - Perturbation Biology) Foster City, CA $226,185.00-$292,710.00 2 days agoWe're unlocking community knowledge in a new way. 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