Required Skills & Experience
- BA, MS, or PHD in synthetic biology, molecular biology, bioengineering, cell biology or related field with wet lab experience in a mammalian biology context.
- Experience with upstream viral vector production protocols including transfection, harvest, and ultracentrifuge or column-based purification (**AAV production experience)
- DD PCR (Digital Droplet PCR)
- Experience with mammalian cell production
- Advanced experience with viral vector production
- SOP revision and creation
Nice to Have Skills & Experience
- ELISA experience
- Western Blot Experience
- Experience performing viral transductions, flow cytometry, and quantitative gene expression analyses.
- Familiarity with liquid handling automation systems for mammalian cell culture.
Job Description
An employer is looking for a Senior Research Associate, Viral Vector Analytics Support scientists in establishing small and mid-scale AAV production processes with mammalian cell lines. This will enable pharmaceutical/biotech organizations to bring more cell and gene therapies to patients, by dramatically increasing the efficiency of viral vector production and reducing the cost of manufacturing.
Other Responsibilities Include
- This role will support the development and execution of workflows for AAV characterization including titer, packaging ratio, aggregation, and sequence integrity.
- Perform routine sterile cell culture work for mammalian cell lines, including cell passage and cell banking.
- Contribute to the writing and maintenance of SOPs.
- Communicate and present experimental and project progress to the Research team.
- Collaborate with Operations and other teams at 64x Bio to secure experimental resources to complete experiments on time.
- Create AAV title, take the cell line from genome engineering team, genome engineering, SOP is there, develop the process, and mainly be execution focused (not as DOE focused).
Location: Brisbane, CA, 94005 (fully onsite)
Contract: 6-12 months, extension or conversion based on candidate performance
Pay: $45-$55/hr
- Candidate pay is based on multiple factors including experience